Experimental principle of human interleukin 8 (IL-8 / CXCL8) ELISA kit
1. The level of human interleukin 8 (IL-8 / CXCL8) ELISAKit in the specimens was measured by double antibody sandwich method.
2. First coat the microplate with purified human interleukin 8 (IL-8 / CXCL8) ELISAKit to make a solid phase antibody.
3. Then add interleukin 8 (IL-8 / CXCL8) ELISAKit to the microwells coated with mAb in turn, and then combine with HRP labeled interleukin 8 (IL-8 / CXCL8) ELISAKit antibody to form antibody-antigen-enzyme labeled antibody After thorough washing, the complex is added with substrate TMB for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.
4. The color depth is positively correlated with the interleukin-8 (IL-8 / CXCL8) ELISAKit in the sample.
5. Finally, the absorbance (OD value) is measured with a microplate reader at a wavelength of 450 nm, and the concentration of human L-interleukin 8 (IL-8 / CXCL8) ELISAKit in the sample is calculated from the standard curve
.
Objective: To determine the content of interleukin-8 (IL-8 / CXCL8) ELISAKit in human serum, plasma and related liquid samples.
6. Serum: Blood coagulates naturally at room temperature for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins.
7. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate.
safety:
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.
4 Keep Elisa kit away from children
Bring your own items:
1. 37 ℃ incubator
2. Standard specification microplate reader
3. Precision pipette and disposable tip
4. Distilled water
5. Disposable test tube
6. Absorbent paper
Storage conditions and validity period:
1. Kit storage :; 2-8 ℃.
2. Validity: 6 months
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