As the most widely used natural medium in vitro, serum is widely used in ELISA kit experiments. So, do you know the specific role of serum in cell culture media? Let's take a look at the instructions for the China Elisa Kit sales platform.
Serum plays a multifaceted role in animal cell culture:
1 Provides growth regulators necessary for cell survival and proliferation.
2 Supplement the nutrients in the basal medium without or insufficient amount.
3 contains some matrix components for adherent cell type cells to adhere to the wall.
4 provides carrier protein, which can bind vitamins, lipids, metal ions and the like.
5 In some cases, neutralizing toxic substances protect cells from damage.
6 Provide a good buffer system for the culture solution.
7Protease inhibitors are provided to protect cells from proteases released by dead cells.
However, serum also has some drawbacks, such as the existence of many harmful components (complement, immunoglobulin and some growth inhibitory factors, etc.); serum components are not clear, affecting the analysis of results; different animals, different batches of serum components and activities have Very different, making the culture results unstable.
However, serum is still the most basic additive in the culture medium, especially in the primary culture or poor cell growth state, it is often cultured first with serum culture medium, and then replaced with serum-free culture after the cells grow vigorously. liquid.
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