Use and maintenance of biological safety cabinets:
1. Purpose: To standardize the operation and maintenance of the biological safety cabinet to ensure the normal operation of the equipment to ensure the safety of the operators.
2. Scope: BHC-1300A2 biosafety cabinet used by aseptic laboratory positive bacteria inspectors.
3. Responsibilities: The bacteria inspector is responsible for the normal operation and maintenance of the equipment, and the quality manager reviews the relevant documents.
4. Content 1 The safety cabinet should be placed in a primary clean room below 100,000. Before operation, the UV lamp should be turned on for 30 minutes, then all the items needed for this operation should be moved into the safety cabinet to avoid frequent breakage of the airflow through the air curtain; and the surface of the object should be disinfected with 75% alcohol before being removed to remove Pollution.
2 Turn on the fan for 10 minutes, and then carry out the experimental operation after the air in the cabinet is purified and the airflow is stable. Slowly extend your arms into the safety cabinet, at least for 2 minutes, and then stabilize the airflow in the cabinet before operating.
3 Items not related to this experiment are not placed in the biosafety cabinet. Items should be placed as far back as possible, and the airway opening should not be blocked to avoid interference with normal airflow.
4 Avoid cross contamination during operation. To prevent possible spills, prepare a 75% alcohol cotton ball or a small piece of gauze soaked in a disinfectant to avoid covering the grille of the safety cabinet with items.
5 During the experimental operation, the glass window should not be fully opened, and the operator's face should be above the working window. When operating in the cabinet, the action should be gentle and soothing to prevent airflow in the cabinet.
6 The safety cabinet should be inspected and maintained regularly to ensure its normal operation. Once the safety cabinet is found to be working abnormally, stop working immediately, take appropriate measures, and notify the quality manager.
7 After the inspection is completed, close the glass window, keep the fan running for 10 minutes, and turn on the UV lamp for 30 minutes.
8 The safety cabinet should be cleaned and disinfected regularly. The surface of the work surface and the outer surface of the cabinet can be wiped with 75% alcohol or 0.2% benzalkonium chloride solution; it should be completely disinfected after each inspection work.
9 The items used in the cabinet should be taken out after disinfection in the disinfection tank to prevent the residual of the standard strains from being polluted and polluting the environment, resulting in biological hazards.
10 How to use:
1) The safety cabinet is controlled by LED liquid crystal panel. The function keys included in the control panel are: power on key, germicidal light button, windshield button (fast, slow, no wind), lighting button.2) After plugging in the 220V AC power supply, press the power switch shown on the control panel to turn it on. When sterilization is required, press the sterilization light button; the windshield key has three levels (fast, slow, no wind) cycle. The default is no wind, press once for slow speed, then press for fast, the third time for no wind, so cycle; usually operate the lighting, just press the button.
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