Pipettes are essential tools in laboratories, widely used by scientists across various fields such as biology, pharmaceuticals, and chemistry. Researchers often prioritize the durability and accuracy of pipettes, as these factors directly impact the reliability of their experiments. To help you choose the right pipette, use it correctly, extend its lifespan, and ensure accurate liquid handling, Lebee starts with the fundamentals of pipettes. This introductory guide covers basic knowledge that forms the foundation for more advanced concepts. Though brief, this information is crucial for understanding future topics. Please take your time to read through it carefully.
The working principle of a pipette is straightforward: a spring-driven piston moves up and down to either draw in or expel liquid. This mechanism allows for precise control over the volume being transferred.
A typical pipette consists of several key components: a control button (used for adjusting the volume), a suction button, a volume display window, a sleeve, an elastic tip, and a pipette head. These parts work together to ensure smooth and accurate operation.
There are two main pipetting technologies designed to meet the growing demand for precision when handling different types of liquids:
1. **Built-in Piston Pipetting Mode** – This is the most common type found in laboratories. The piston is housed inside the pipette sleeve, with an air gap between the piston and the liquid. This design prevents direct contact between the liquid and the internal components. However, this also means that if the sample contaminates the piston, cross-contamination can occur. While convenient and widely used, this method is not ideal for highly viscous or volatile liquids. Improper use can lead to inaccuracies or even internal damage to the pipette.
2. **External Piston Pipetting Mode** – Designed for handling viscous or sensitive liquids, this type places the piston outside the pipette sleeve, inside the disposable tip. There is no air gap between the piston and the liquid, which minimizes the risk of aerosol contamination. This makes it particularly suitable for handling valuable reagents, biological samples, and other delicate materials. The disposable piston ensures cleanliness and accuracy, making it a preferred choice in many specialized applications.
Both methods have their own advantages and limitations, and choosing the right one depends on the specific requirements of your experiment. Understanding how each works can significantly improve your lab efficiency and the quality of your results.
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