Pipettes are essential tools in laboratories, widely used by scientists across various fields such as biology, pharmaceuticals, and chemistry. When selecting a pipette, users typically focus on its durability and accuracy. To help you choose the right one, use it correctly, extend its lifespan, and ensure precise measurements, Lebee starts with the fundamentals of pipettes. This introduction is the first step in understanding more advanced topics. Though brief, this content serves as a solid foundation for future learning. We encourage you to read it carefully and take your time.
The working principle of a pipette is straightforward: it uses a spring mechanism to move a piston up and down, allowing liquid to be drawn in or expelled. This simple yet effective design makes pipettes reliable instruments in laboratory settings.
A typical pipette consists of several key components: a volume adjustment button (used to set the desired volume), a suction button, a display window for viewing the volume, a sleeve, an elastic tip, and a pipette head. These parts work together to ensure accurate and controlled liquid handling.
There are two main pipetting technologies designed to meet different requirements for precision and sample compatibility:
1. **Built-in Piston Pipetting** – This is the most common type found in laboratories. The piston is located inside the pipette sleeve, with an air gap between the piston and the liquid. This design prevents direct contact between the liquid and the internal mechanisms, reducing the risk of contamination. However, it has some limitations. It is not ideal for highly viscous or volatile liquids, and improper handling can lead to cross-contamination or reduced accuracy. Additionally, if the piston becomes contaminated, it may cause damage to the pipette over time.
2. **External Piston Pipetting** – Designed for more challenging samples, this type features a piston that is located outside the pipette body, within the disposable tip. There is no air gap between the piston and the liquid, which minimizes the risk of aerosol contamination. This makes it particularly suitable for handling sensitive biological samples or expensive reagents. It also performs better with viscous liquids, offering greater precision and reliability in such cases.
Both types have their advantages and are chosen based on the specific needs of the experiment. Understanding these differences helps in making informed decisions about which pipette to use for different applications.
Whether you're a seasoned researcher or just starting out, mastering the basics of pipette operation is crucial for maintaining accuracy and efficiency in your lab work. Take the time to learn and practice, and you'll find that even the simplest tools can make a big difference in your results.
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