English name: Fast green FCF; Food green 3; CI 42053 Other names: Fast green FCF; Fast green; Fast green CAS number: 2353-45-9 C37H34N2Na2O10S3 = 808.85 Level: High Purity Grade Effective spectroscopic content: ≥90.0% dry Weightlessness: ≤8.0% Em (622nm, 50% Methanol):> 125000 Properties: dark green powder or maroon particles with metallic luster. Hygroscopic. Soluble in water, soluble in ethanol, dark orange in concentrated sulfuric acid, dark green after dilution, orange in concentrated hydrochloric acid or concentrated nitric acid, and bright blue in 10% sodium hydroxide solution. Melting point 290 ° C (decomposition). The maximum absorption wavelength is 628nm. LD50 (rat, oral)> 2G / kG Uses: Biochemical studies. Plant histology and cytology staining. It is a basic dye used for protein staining in IEF, PAGE and SDS-PAGE. Fast green staining covers a wider range of protein concentrations than Brilliant Blue R and is linear. After electrophoresis, the protein is fixed on the gel and stained more sensitively. The gel is stained with 0.1% fast green in 30% (v / v) ethanol, 10% (v / v) acetic acid, or 7% acetic acid for 2 hours and then 30 % (v / v) ethanol, 10% (v / v) acetic acid or 7% acetic acid to de-stain. Stained glue can be viewed at 625 nm. The detection sensitivity is 30% higher than Brilliant Blue R (Brilliant Blue R). In addition, fast green is also used for sulfite determination, manufacturing bacterial culture medium, used to identify Escherichia coli and other lactose-fermenting bacteria, culture and separation of typhoid bacillus in feces. Storage: RT
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