1. The human ELISA kit should be taken out from the cold environment and equilibrated at room temperature for 15-30 minutes before use.
After use, the slats should be stored in sealed bags.
2. Crystals may be precipitated in the concentrated washing liquid, which can be heated and dissolved in the water bath during dilution, and the results will not be affected during washing.
3. The sample adder should be used in each step of sample addition, and its accuracy should be regularly checked to avoid test errors. One sample loading time is best
Control within 5 minutes, if the number of specimens is large, it is recommended to use a volley gun to add samples.
4. Please make a standard curve every time when measuring the human ELISA kit, and it is best to do the multi-well. If the content of the test substance in the specimen is too high (sample OD value
Is greater than the OD value of the first well of the standard product), please dilute it with a certain multiple (n times) of the sample diluent before measuring.
When calculating, please multiply the total dilution factor (× n × 5).
5. The sealing film is limited to one-time use to avoid cross contamination.
6. Please keep the substrate away from light.
7. Strictly follow the instructions, and the test results must be determined based on the reading of the microplate reader.
8. All samples, washing liquid and various wastes should be treated as infectious agents.
9. The components of different batches of my ELISA kit must not be mixed.
1. Kit storage :; 2-8 ℃.
2. Validity: 6 months
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