How to remove mycoplasma from cells

1, with MRA treatment

The cells were treated with Mycoplasma Removal Agent and changed every 4 days for 15 days to ensure the cells were pure and healthy.

2. Method for removing mycoplasma contamination by washing and purifying method

Cell nutrition and domestication → screening of high quality cell population → cell washing → repeated centrifugation

The principle is to use the centrifugal force, cell, microbial quality and the buoyancy difference of the suspension to achieve the purpose of removing mycoplasma. Because the mycoplasma individuals are small and the extracellular parasites are mostly in vitro, the number of potential mycoplasmas is reduced to the limit by repeated washing of cells and low-speed centrifugation.

If combined with the anti-killing effect of sensitive antibiotics, better results can be achieved.

3, drug-assisted heating treatment

After treatment with the drug, the contaminated tissue culture was cultured at 41 ° C for 18 hours to kill mycoplasma, but had adverse effects on the cells.

4. Use mycoplasma-specific serum

§ 5% of rabbit mycoplasma immune serum can remove mycoplasma contamination, because specific antibodies can inhibit the growth of mycoplasma, so it was negative after 11 days of antiserum treatment, and still negative after 5 months.

Shanghai Hengyuan Biotechnology Co., Ltd. stocks a variety of cell lines, primary cells, passage cells, delivery time of about 2 weeks, to ensure cell quality, problems can be reissued, customers are welcome to consult and order

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